DOI: 10.1016/j.apsusc.2020.146311

 

    In order to improve bone growth and imitate the function of natural extracellular matrix to continuously release multiple growth factors, electrospinning was used to prepare porous gelatin nanofiber scaffolds, hydroxyapatite was coated with a simulated body fluid solution, and avidin was used for surface function The ratio of bone morphogenetic protein 2 (BMP-2) and fibroblast growth factor 2 (FGF-2) is different. Scanning electron microscopy was used for structural characterization, and Fourier transform infrared spectroscopy and calcium determination were performed to evaluate the composition of calcium. The immunostaining technique confirmed the combination of multiple growth factors. Quantitative polymerase chain reaction analysis was used to study the effects of two growth factors and hydroxyapatite on the differentiation of osteoblasts. By controlling the FGF-2/BMP-2 ratio, multiple growth factors were successfully conjugated to the functionalized surface. The comparison of the factor release curve and the physical adsorption curve shows that the avidin-biotin combination is more effective for sustained release. Bone regeneration is enhanced by the synergy between the delivery of multiple growth factors and the hydroxyapatite nanofiber coating, as evidenced by the increased expression of osteogenic gene markers. Therefore, nanofibers provide a promising osteoconductive scaffold material with controlled release of multiple growth factors for bone tissue engineering.

 

    Article source: Yisibang

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